Antifungal Activity of Saponins from the Fruit Pericarp of Sapindus mukorossi against Venturia inaequalis and Botrytis cinerea

01/05/2018

Authors

F. M. Porsche, D. Molitor, M. Beyer, S. Charton, C. André, and A. Kollar

Reference

Plant Disease, vol. 102, no. 5, pp. 991-1000, 2018

Description

The antifungal activity of an aqueous extract (AE) and the solid fraction of a chloroform-methanol fruit pericarp extract (CME) of Sapindus mukorossi resolved in water was tested for the first time against Venturia inaequalis and Botrytis cinerea – two important fungal pathogens worldwide. In the greenhouse, a CME (1% v/v) spray significantly reduced V. inaequalis symptoms and sporulation (99%) on apple seedling leaves (P ≤ 0.05). In field trials, applications of AE (1% v/v) reduced the disease severity of B. cinerea on grapes on average by 63%. Extracts were fractionated by HPLC and the bioefficacy of the fractions was tested in vitro. Some components of the most fungicidal fraction were identified by liquid chromatography-high resolution mass spectrometry as saponins: sapindoside B (accounting for ≥ 98% of the total constituents), hederagenin-pentosylhexoside, and oleanolic acid-hexosyl-deoxyhexosyl-hexoside. This fraction inhibited the mycelial growth of V. inaequalis and B. cinerea by 45% and 43%, respectively.

Link

doi:10.1094/PDIS-06-17-0906-RE

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