Optimizing peptide detection using FET-based sensors: Integrating non-linearities of surface functionalization
Authors
Kumar N., Dixit A., Kumar P., Ansari M.H.R., Bagga N., Gandhi N., Kondekar P.N., García C.P., Georgiev V.
Reference
Solid State Electronics, vol. 229, art. no. 109161, 2025
Description
This study presents a method for enhancing peptide interaction with ATPES/linkers, utilizing tert-Butyloxycarbonyl to protect the N-terminal and prevent undesirable reactions. Addressing noise interference in peptide recognition, we devised a noise-filtering technique that enables accurate peptide signature retrieval even at low signal-to-noise ratio (SNR) by leveraging the full pH titration spectrum. Additionally, our research explores the impact of BOC protection levels on the functionalization of amine surfaces with APTES, highlighting how silanol sites influence the isoelectric point, surface potential, and capacitance. These findings underscore the necessity of considering chemical protection and surface chemistry in peptide synthesis and sequencing, offering valuable insights for bioanalytical applications.
