Type de contrat: Stage
Durée: 6 months
Lieu de travail: Belvaux
The Luxembourg Institute of Science and Technology (LIST) is a mission-driven Research and Technology Organization (RTO) that develops advanced technologies and delivers innovative products and services to industry and society. As a major engine of the diversification and growth of Luxembourg’s economy through innovation, LIST supports the deployment of a number of solutions to a wide range of sectors, including energy, IT, telecommunications, environment, agriculture, and advanced manufacturing at national and European level. Thanks to its location in an exceptional collaborative environment, namely the Belval Innovation Campus, LIST accelerates time to market by maximizing synergies with different actors, including the university, the national funding agency and industrial clusters.
The Green Tech platform is running analyses in different omics and analytical chemistry on environmental samples, from plants to water. Biotechnologies are also key in the processes of molecules of interest.
In the frame of the SMARTWALL project, on the impact of dehydration/rehydration cycle on resurrection plants, different types of analyses are required to study the proteome and the metabolome of resurrection plant cells. Undifferentiated cells have been habituated to cellulose biosynthesis inhibitors by growing them on stepwise increasing concentrations of dichlobenil and isoxaben and they will be treated with 200 mM mannitol, which mimicks drought stress. Cells will be collected and would require an in-depth analysis.
The tasks will consist in the extraction of proteins for their analysis by LC-MSMS and/or 2D DIGE. A metabolomics study will also be conducted for these samples. Furthermore, results will be compiled and analysed by bioinformatics tools.
Data will be used for further publications (poster or manuscripts).